Genetic similarity between parents predicts hatching failure: non-incestuous inbreeding in the great reed warbler

Bensch, S., Hasselquist, D. & von Schantz, T.
Evolution 48:317-326. 1994.


Abstract
The DNA fingerprinting technique was used to find the true pedigrees and to detect the overall genetic similarity between mates of great reed warblers (Acrocephalus arundinaceus) at an isolated breeding site in Sweden. The study covered four years preceded by three years when almost all adults and nestlings in the study area had been ringed. DNA fingerprinting revealed that the putative father had sired the overwhelming majority of the young. The mate's genetic similarity, revealed as the proportion of bands shared in RFLP patterns, was high compared to other species of wild birds. Also, band sharing was higher between mates native to the area than between pairs where the female was experimentally introduced from a distant breeding site. Hatching success of eggs was negatively correlated with the degree of genetic similarity between the mates despite the fact that pedigree data suggest outbreeding. These are the first data showing that there is a significant fitness cost associated with the choice of a mate which has high genetic similarity, even if it is not a close kin. This cost might be due to generalized deleterious consequences of genome-wide inbreeding, in the present study possibly accentuated by recent population bottlenecks.